Proteintech, 67750-1-Ig, RUVBL1 Monoclonal antibody

Size: 20ul / 150ul The RUVBL1 (67750-1-Ig) by Proteintech is a Monoclonal antibody targeting RUVBL1 in WB, ELISA applications with reactivity to Human, rat samples 67750-1-Ig targets RUVBL1 in WB, ELISA applications and shows reactivity with Human, rat samples. Tested Applications Positive WB detected in: U2OS cells, HeLa cells, HT-1080 cells, HCT 116 cells, K-562 cells, A549 cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. Specification Tested Reactivity: Human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16688 Product name: Recombinant human RUVBL1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-214 aa of BC002993 Sequence: MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFD Predict reactive species Full Name: RuvB-like 1 (E. coli) Calculated Molecular Weight: 456 aa, 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC002993 Gene Symbol: RUVBL1 Gene ID (NCBI): 8607 RRID: AB_2918519 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9Y265 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.