Proteintech, 67751-1-Ig, PRKAR2A Monoclonal antibody

Size: 20ul / 150ul The PRKAR2A (67751-1-Ig) by Proteintech is a Monoclonal antibody targeting PRKAR2A in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, rat, mouse samples 67751-1-Ig targets PRKAR2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples. Tested Applications Positive WB detected in: U2OS cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, HepG2 cells, HeLa cells, LNCaP cells Positive IHC detected in: human colon cancer tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. Specification Tested Reactivity: Human, rat, mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag29893 Product name: Recombinant human PRKAR2A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 74-382 aa of BC002763 Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ Predict reactive species Full Name: protein kinase, cAMP-dependent, regulatory, type II, alpha Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC002763 Gene Symbol: PRKAR2A Gene ID (NCBI): 5576 RRID: AB_2918520 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P13861 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.