Proteintech, 67766-1-Ig, gp130/IL6ST Monoclonal antibody

Size: 20ul / 150ul The gp130/IL6ST (67766-1-Ig) by Proteintech is a Monoclonal antibody targeting gp130/IL6ST in WB, IHC, ELISA applications with reactivity to human samples 67766-1-Ig targets gp130/IL6ST in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, NCCIT cells, PC-3 cells, DU 145 cells Positive IHC detected in: human placenta tissue, human colon cancer tissue, human liver cancer tissue, human lung cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28808 Product name: Recombinant human IL-6ST protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 124-369 aa of NM_002184 Sequence: MLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVT Predict reactive species Full Name: interleukin 6 signal transducer (gp130, oncostatin M receptor) Calculated Molecular Weight: 104 kDa Observed Molecular Weight: 130-140 kDa GenBank Accession Number: NM_002184 Gene Symbol: IL6ST Gene ID (NCBI): 3572 RRID: AB_2918533 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P40189 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.