Proteintech, 67805-1-Ig, USP11 Monoclonal antibody

Size: 20ul / 150ul The USP11 (67805-1-Ig) by Proteintech is a Monoclonal antibody targeting USP11 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 67805-1-Ig targets USP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Ramos cells, Daudi cells Positive IHC detected in: rat testis tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag19058 Product name: Recombinant human USP11 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 441-743 aa of BC000350 Sequence: YLSVPLLISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVRLQECI Predict reactive species Full Name: ubiquitin specific peptidase 11 Calculated Molecular Weight: 963 aa, 110 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC000350 Gene Symbol: USP11 Gene ID (NCBI): 8237 RRID: AB_2918568 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P51784 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.