Proteintech, 67826-1-Ig, Phospho-P53 (Ser15) Monoclonal antibody

Size: 20ul / 100ul The Phospho-P53 (Ser15) (67826-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-P53 (Ser15) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 67826-1-Ig targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Calyculin A treated HT-29 cells, HT-29 cells, HEK-293 cells, HEK-293t cells, Etoposide treated HT-29 cells, Calyculin A treated HEK-293 cells, UV treated HEK-293T cells Positive IF/ICC detected in: etoposide treated HT-29 cells Positive FC (Intra) detected in: UV treated HT-29 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tumor protein p53 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC003596 Gene Symbol: P53 Gene ID (NCBI): 7157 RRID: AB_2918588 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P04637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.