Proteintech, 67885-1-Ig, CBFB Monoclonal antibody

Size: 20ul / 150ul The CBFB (67885-1-Ig) by Proteintech is a Monoclonal antibody targeting CBFB in WB, FC (Intra), ELISA applications with reactivity to human, rat samples 67885-1-Ig targets CBFB in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HEK-293 cells, MOLT-4 cells, K-562 cells, HSC-T6 cells Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa. Specification Tested Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag31183 Product name: Recombinant human CBFB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-182 aa of BC018509 Sequence: MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTTRP Predict reactive species Full Name: core-binding factor, beta subunit Calculated Molecular Weight: 182 aa, 22 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: BC018509 Gene Symbol: CBFB Gene ID (NCBI): 865 RRID: AB_2918642 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q13951 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.