Proteintech, 67890-1-Ig, HSPB3 Monoclonal antibody

Size: 20ul / 150ul The HSPB3 (67890-1-Ig) by Proteintech is a Monoclonal antibody targeting HSPB3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, rabbit samples 67890-1-Ig targets HSPB3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples. Tested Applications Positive WB detected in: human heart tissue, mouse heart tissue, rabbit heart tissue, rat heart tissue Positive IHC detected in: rat kidney tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa. Specification Tested Reactivity: human, mouse, rat, rabbit Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag26940 Product name: Recombinant human HSPB3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-130 aa of BC126272 Sequence: MAKIILRHLIEIPVRYQEEFEARGLEDCRLDHALYALPGPTIVDLRKTRAAQSPPVDSAAETPPREGKSHFQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFISRSFTRQYKLPDGVEIKDLS Predict reactive species Full Name: heat shock 27kDa protein 3 Calculated Molecular Weight: 150 aa, 17 kDa Observed Molecular Weight: 20 kDa GenBank Accession Number: BC126272 Gene Symbol: HSPB3 Gene ID (NCBI): 8988 RRID: AB_2918646 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q12988 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.