Proteintech, 67893-1-Ig, GARS Monoclonal antibody

Size: 20ul / 150ul The GARS (67893-1-Ig) by Proteintech is a Monoclonal antibody targeting GARS in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse, rat samples 67893-1-Ig targets GARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, HepG2 cells, HeLa cells，HEK-293 cells，HepG2 cells，Jurkat cells，K-562 cells，HSC-T6 cells，NIH/3T3 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa. Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8432 Product name: Recombinant human GARS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 390-739 aa of BC007755 Sequence: MRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE Predict reactive species Full Name: glycyl-tRNA synthetase Calculated Molecular Weight: 739 aa, 83 kDa Observed Molecular Weight: 75-80 kDa GenBank Accession Number: BC007755 Gene Symbol: GARS Gene ID (NCBI): 2617 RRID: AB_2918649 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P41250 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.