Proteintech, 67898-1-Ig, Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody

Size: 20ul / 100ul The Phospho-S6 Ribosomal protein (Ser235) (67898-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-S6 Ribosomal protein (Ser235) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 67898-1-Ig targets Phospho-S6 Ribosomal protein (Ser235) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, IGF-1 treated MCF-7 cells, Calyculin A treated NIH/3T3 cells Positive IF/ICC detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells Positive FC (Intra) detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ribosomal protein S6 Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC000524 Gene Symbol: RPS6 Gene ID (NCBI): 6194 RRID: AB_2918654 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P62753 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.