Proteintech, 67900-1-Ig, Phospho-P53 (Ser46) Monoclonal antibody

Size: 20ul / 100ul The Phospho-P53 (Ser46) (67900-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-P53 (Ser46) in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 67900-1-Ig targets Phospho-P53 (Ser46) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-29 cells, Calyculin A treated HT-29 cells, etoposide treated HT-29 cells, UV treated A431 cells, Calyculin A treated HEK-293 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: etoposide treated HT-29 cells Positive FC (Intra) detected in: HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information P53 is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tumor protein p53 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC003596 Gene Symbol: P53 Gene ID (NCBI): 7157 RRID: AB_2918656 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P04637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.