Proteintech, 67901-1-Ig, CIB1 Monoclonal antibody

Size: 20ul / 150ul The CIB1 (67901-1-Ig) by Proteintech is a Monoclonal antibody targeting CIB1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, bovine samples 67901-1-Ig targets CIB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, bovine samples. Tested Applications Positive WB detected in: LNCaP cells, Hela cells, K-562 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Human milk Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Positive FC (Intra) detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse, rat, bovine Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag31174 Product name: Recombinant human CIB1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-191 aa of BC000846 Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL Predict reactive species Full Name: calcium and integrin binding 1 (calmyrin) Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: BC000846 Gene Symbol: CIB1 Gene ID (NCBI): 10519 RRID: AB_2918657 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99828 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.