Proteintech, 67928-1-Ig, PSME1 Monoclonal antibody

Size: 20ul / 150ul The PSME1 (67928-1-Ig) by Proteintech is a Monoclonal antibody targeting PSME1 in WB, IF/ICC, ELISA applications with reactivity to Human, mouse, rat samples 67928-1-Ig targets PSME1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, Hela cells, HEK-293 cells, Jurkat cells, k-562 cells, HSC-T6 cells, C6 cells, NIH/3T3 cells and 4T1 cells. Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30549 Product name: Recombinant human PSME1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-249 aa of BC007503 Sequence: MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY Predict reactive species Full Name: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 29 kDa GenBank Accession Number: BC007503 Gene Symbol: PSME1 Gene ID (NCBI): 5720 RRID: AB_2918680 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q06323 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.