Proteintech, 67952-1-Ig, TrpRS Monoclonal antibody

Size: 20ul / 150ul The TrpRS (67952-1-Ig) by Proteintech is a Monoclonal antibody targeting TrpRS in WB, IF/ICC, ELISA applications with reactivity to Human, rat, mouse samples 67952-1-Ig targets TrpRS in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples. Tested Applications Positive WB detected in: LNCaP cells, HSC-T6 cells, 4T1 cells, human placenta tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IF/ICC detected in: LNCaP cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684). Specification Tested Reactivity: Human, rat, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag31430 Product name: Recombinant human WARS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-368 aa of BC017489 Sequence: MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQI Predict reactive species Full Name: tryptophanyl-tRNA synthetase Calculated Molecular Weight: 471 aa, 53 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC017489 Gene Symbol: WARS1 Gene ID (NCBI): 7453 RRID: AB_2918704 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P23381 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.