Proteintech, 67986-1-Ig, FAH Monoclonal antibody

Size: 20ul / 150ul The FAH (67986-1-Ig) by Proteintech is a Monoclonal antibody targeting FAH in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 67986-1-Ig targets FAH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: LNCaP cells, pig liver tissue, rat liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6704 Product name: Recombinant human FAH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 70-419 aa of BC002527 Sequence: FMGLGQAAWKEARVFLQNLLSVSQARLRDDTELRKCAFISQASATMHLPATIGDYTDFYSSRQHATNVGIMFRDKENALMPNWLHLPVGYHGRASSVVVSGTPIRRPMGQMKPDDSKPPVYGACKLLDMELEMAFFVGPGNRLGEPIPISKAHEHIFGMVLMNDWSARDIQKWEYVPLGPFLGKSFGTTVSPWVVPMDALMPFAVPNPKQDPRPLPYLCHDEPYTFDINLSVNLKGEGMSQAATICKSNFKYMYWTMLQQLTHHSVNGCNLRPGDLLASGTISGPEPENFGSMLELSWKGTKPIDLGNGQTRKFLLDGDEVIITGYCQGDGYRIGFGQCAGKVLPALLPS Predict reactive species Full Name: fumarylacetoacetate hydrolase (fumarylacetoacetase) Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC002527 Gene Symbol: FAH Gene ID (NCBI): 2184 RRID: AB_2918735 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P16930 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.