Proteintech, 67988-1-Ig, RAD23B Monoclonal antibody

Size: 20ul / 150ul The RAD23B (67988-1-Ig) by Proteintech is a Monoclonal antibody targeting RAD23B in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, pig samples 67988-1-Ig targets RAD23B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, pig brain tissue, HEK-293 cells, Huh-7 cells, human platelet cells, rat brain tissue, mouse brain tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58). Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28658 Product name: Recombinant human RAD23B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-409 aa of BC020973 Sequence: MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAVSTPAPATTQQSAPASTTAVTSSTTTTVAQAPTPVPALAPTSTPASITPASATASSEPAPASAAKQEKPAEKPAETPVATSPTATDSTSGDSSRSNLFEDATSALVTGQSYENMVTEIMSMGYEREQVIAALRASFNNPDRAVEYLLMGIPGDRESQAVVDPPQAASTGVPQSSAVAAAAATTTATTTTTSSGGHPLEFLRNQPQFQQMRQIIQQNPSLLPALLQQIGRENPQLLQQISQHQEHFIQMLNEPVQEAGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEKNENLAANFLLQQNFDED Predict reactive species Full Name: RAD23 homolog B (S. cerevisiae) Calculated Molecular Weight: 409 aa, 43 kDa Observed Molecular Weight: 58 kDa GenBank Accession Number: BC020973 Gene Symbol: RAD23B Gene ID (NCBI): 5887 RRID: AB_2918737 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P54727 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.