Proteintech, 67989-1-Ig, MCM6 Monoclonal antibody

Size: 20ul / 150ul The MCM6 (67989-1-Ig) by Proteintech is a Monoclonal antibody targeting MCM6 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, Rat, Mouse samples 67989-1-Ig targets MCM6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, HEK-293 cells, COLO 320 cells, HepG2 cells, MCF-7 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000 Background Information The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa. Specification Tested Reactivity: Human, Rat, Mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4354 Product name: Recombinant human MCM6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 471-821 aa of BC032374 Sequence: VAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED Predict reactive species Full Name: minichromosome maintenance complex component 6 Calculated Molecular Weight: 821 aa, 93 kDa Observed Molecular Weight: 105 kDa GenBank Accession Number: BC032374 Gene Symbol: MCM6 Gene ID (NCBI): 4175 RRID: AB_2918738 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q14566 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.