Proteintech, 68051-1-Ig, GAS2L1 Monoclonal antibody

Size: 20ul / 150ul The GAS2L1 (68051-1-Ig) by Proteintech is a Monoclonal antibody targeting GAS2L1 in WB, IHC, ELISA applications with reactivity to Human, Mouse samples 68051-1-Ig targets GAS2L1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: Jurkat cells, mouse testis tissue, K-562 cells, NIH/3T3 cells, 4T1 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950). Specification Tested Reactivity: Human, Mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag31232 Product name: Recombinant human GAS2L1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-329 aa of BC011047 Sequence: MADPVAGIAGSAAKSVRPFRSSEAYVEAMKEDLAEWLNALYGLGLPGGGDGFLTGLATGTTLCQHANAVTEAARALAAARPARGVAFQAHSVVPGSFMARDNVATFIGWCRVELGVPEVLMFETEDLVLRKNEKSVVLCLLEVARRGARLGLLAPRLVQFEQEIERELRAAPPAPNAPAAGEDTTETAPAPGTPARGPRMTPSDLRNLDELVREILGRCTCPDQFPMIKVSEGKYRVGDSSLLIFVRVLRSHVMVRVGGGWDTLEHYLDKHDPCRCSSTAHRPPQPRVCTFSPQRVSPTTSPRPASPVPGSERRGSRPEMTPVSLRSTK Predict reactive species Full Name: growth arrest-specific 2 like 1 Calculated Molecular Weight: 73 kDa Observed Molecular Weight: 73 kDa GenBank Accession Number: BC011047 Gene Symbol: GAS2L1 Gene ID (NCBI): 10634 RRID: AB_2918793 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q99501 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.