Proteintech, 68055-1-Ig, m6A Monoclonal antibody

Size: 20ul / 150ul The m6A (68055-1-Ig) by Proteintech is a Monoclonal antibody targeting m6A in IHC, RIP, Dot Blot, ELISA applications with reactivity to chemical compound, m6a samples 68055-1-Ig targets m6A in WB, IHC, IF, IP, ChIP, RIP, Dot Blot, ELISA applications and shows reactivity with chemical compound, m6a samples. Tested Applications Positive RIP detected in: RNA Positive IHC detected in: mouse testis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, rat lymph nodeNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive ELISA detected in: m6A Positive Dot Blot detected in: RNA Recommended dilution RIP: RIP : 5-10μg antibody/mg lysate Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:20000 DOT BLOT: DOT BLOT : 1:1000-1:4000 Background Information m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.Protocol for Dot Blot:https://www.ptglab.com/protocol/68055-1-IgDotBlot.pdf Specification Tested Reactivity: chemical compound, m6a Cited Reactivity: human, mouse, rat, pig, monkey Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: m6A GenBank Accession Number: m6A RRID: AB_2918796 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.