Proteintech, 68092-1-Ig, MIST1 Monoclonal antibody

Size: 20ul / 150ul The MIST1 (68092-1-Ig) by Proteintech is a Monoclonal antibody targeting MIST1 in WB, ELISA applications with reactivity to Human, Pig, Rat , Mouse samples 68092-1-Ig targets MIST1 in WB, ELISA applications and shows reactivity with Human, Pig, Rat , Mouse samples. Tested Applications Positive WB detected in: BxPC-3 cells, SW 1990 cells，Pig pancreas tissue, Rat pancreas tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Muscle intestine stomach expression 1 (MIST1), also named bHLHa15, is a secretory cell transcription factor that belongs to the basic helix-loop-helix superfamily. MIST1 is mainly expressed in serous secreted cells and essential for the development and maturation of secretory cells. Secretory cells lacking MIST1 exhibit structural and functional defects, including alterations in cytoskeletal organization and cell polarity and improper cell-cell junction. In healthy pancreata, MIST1 facilitates cell-to-cell communication, promotes acinar cell polarity, controls Ca2+ flux, promotes unfolded protein response (UPR) homeostasis, aids in vesicle assembly, and is required for regulated exocytosis. MIST1 was downregulated in pancreatic cancer tissues compared with normal or adjacent pancreatic tissues and lower MIST1 expression in pancreatic cancer cell lines than normal acinar cells (BxPC-3, PANC-1, SW 1990) (PMID:29859299). MIST1 is downregulated in the development of gastric cancer and in gastric cancer cells compared with SW620 cells (PMID:34149921). Specification Tested Reactivity: Human, Pig, Rat , Mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18509 Product name: Recombinant human BHLHA15 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-189 aa of BC113394 Sequence: MKTKNRPPRRRAPVQDTEATPGEGTPDGSLPNPGPEPAKGLRSRPARAAARAPGEGRRRRPGPSGPGGRRDSSIQRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLEGPGPKLYQHYQQQQQVAGGALGATEAQPQGHLQRYSTQIHSFREGT Predict reactive species Full Name: basic helix-loop-helix family, member a15 Calculated Molecular Weight: 189 aa, 21 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC113394 Gene Symbol: MIST1 Gene ID (NCBI): 168620 RRID: AB_2918829 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q7RTS1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.