Proteintech, 68124-1-Ig, LPIN1 Monoclonal antibody

Size: 20ul / 150ul The LPIN1 (68124-1-Ig) by Proteintech is a Monoclonal antibody targeting LPIN1 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, Rat, Mouse samples 68124-1-Ig targets LPIN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples. Tested Applications Positive WB detected in: HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells Positive IHC detected in: human liver cancer tissue, rat skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047). Specification Tested Reactivity: Human, Rat, Mouse Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag25813 Product name: Recombinant human LPIN1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 301-460 aa of BC030537 Sequence: ICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRD Predict reactive species Full Name: lipin 1 Calculated Molecular Weight: 890 aa, 99 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC030537 Gene Symbol: LPIN1 Gene ID (NCBI): 23175 RRID: AB_2923652 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q14693 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.