Proteintech, 68125-1-Ig, RAP1A Monoclonal antibody

Size: 20ul / 150ul The RAP1A (68125-1-Ig) by Proteintech is a Monoclonal antibody targeting RAP1A in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples 68125-1-Ig targets RAP1A in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, Raji cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771). Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9463 Product name: Recombinant human RAP1A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-184 aa of BC014086 Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL Predict reactive species Full Name: RAP1A, member of RAS oncogene family Calculated Molecular Weight: 184 aa, 21 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC014086 Gene Symbol: RAP1A Gene ID (NCBI): 5906 RRID: AB_2923653 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P62834 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.