Proteintech, 68129-1-Ig, PFKP Monoclonal antibody

Size: 20ul / 150ul The PFKP (68129-1-Ig) by Proteintech is a Monoclonal antibody targeting PFKP in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, rabbit samples 68129-1-Ig targets PFKP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, rabbit brain tissue, A549 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, rat brain tissue, mouse brain tissue Positive IF/ICC detected in: HeLa cells, BxPC-3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489). Specification Tested Reactivity: human, mouse, rat, rabbit Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag25206 Product name: Recombinant human PFKP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 436-784 aa of BC029138 Sequence: GIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV Predict reactive species Full Name: phosphofructokinase, platelet Calculated Molecular Weight: 784 aa, 86 kDa Observed Molecular Weight: 80-86 kDa GenBank Accession Number: BC029138 Gene Symbol: PFKP Gene ID (NCBI): 5214 RRID: AB_2923656 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q01813 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.