Proteintech, 68130-1-Ig, RYBP Monoclonal antibody

Size: 20ul / 150ul The RYBP (68130-1-Ig) by Proteintech is a Monoclonal antibody targeting RYBP in WB, ELISA applications with reactivity to Human, mouse samples 68130-1-Ig targets RYBP in WB, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: A549 cells, RAW 264.7 cells, HeLa cells, U2OS cells, HEK-293 cells, LNCaP cells, Jurkat cells, Daudi cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914] Specification Tested Reactivity: Human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag31715 Product name: Recombinant human RYBP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-228 aa of BC014959 Sequence: MTMGDKKSPTRPKRQAKPAADEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF Predict reactive species Full Name: RING1 and YY1 binding protein Calculated Molecular Weight: 228 aa, 25 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC014959 Gene Symbol: RYBP Gene ID (NCBI): 23429 RRID: AB_2923657 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q8N488 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.