Proteintech, 68140-1-Ig, ACOT7 Monoclonal antibody

Size: 20ul / 150ul The ACOT7 (68140-1-Ig) by Proteintech is a Monoclonal antibody targeting ACOT7 in WB, ELISA applications with reactivity to Human, Rat, Mouse, Rabbit, Pig samples 68140-1-Ig targets ACOT7 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Acyl-CoA thioesterases (ACOTs) are enzymes that catalyze hydrolysis of fatty acyl-CoAs to free fatty acids and CoA-SH. ACOTs are expressed ubiquitously in prokaryotes and eukaryotes (PMID: 11755680). ACOT7 also known as BACH, CTE-II, ACT, ACH1, BACHa, is one of the most extensively studied ACOTs as a major isoform of the ACOT family (PMID: 17563367). The enzyme is localized to the cytosol and is highly expressed in brain tissue and testis tissue, showing a preference for long-chain acyl-CoA substrates with fatty acid chains of 8-16 carbon atoms (C8-C16) (PMID: 28518146). It has been identified that ACOT7 plays a role in neurotoxic prevention and is a candidate drug target in inflammatory disease (PMID: 17563367, 23459938, 10509865). ACOT7 has 7 isoforms as molecular between 27-40 kDa, it may also form dimer (~70 kDa) (PMID: 20470824). Specification Tested Reactivity: Human, Rat, Mouse, Rabbit, Pig Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8975 Product name: Recombinant human ACOT7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 14-370 aa of BC017365 Sequence: PDTCALLQPPAASAAAAPSMSGPDVETPSAIQICRIMRPDDANVAGNVHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSVEVQVNVMSENILTGAKKLTNKATLWYVPLSLKNVDKVLEVPPVVYSRQEQEEEGRKRYEAQKLERMETKWRNGDIVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVDAINFHDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDSSQKRYRAASAFFTYVSLSQEGRSLPVPQLVPETEDEKKRFEEGKGRYLQMKAKRQGHAEPQP Predict reactive species Full Name: acyl-CoA thioesterase 7 Calculated Molecular Weight: 370 aa, 40 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC017365 Gene Symbol: ACOT7 Gene ID (NCBI): 11332 RRID: AB_2923667 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O00154 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.