Product Description
Size: 20ul / 150ul
The EEF1G (68148-1-Ig) by Proteintech is a Monoclonal antibody targeting EEF1G in WB, IF/ICC, ELISA applications with reactivity to human, pig, rabbit samples
68148-1-Ig targets EEF1G in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.
Tested Applications
Positive WB detected in: U2OS cells, HeLa cells, MCF-7 cells, pig heart tissue, A549 cells, HEK-293 cells, rabbit heart tissue, pig brain tissue, rabbit brain tissue
Positive IF/ICC detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
Background Information
Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
Specification
Tested Reactivity: human, pig, rabbit
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag0218 Product name: Recombinant human EEF1G protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 140-400 aa of BC006520 Sequence: GLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR Predict reactive species
Full Name: eukaryotic translation elongation factor 1 gamma
Calculated Molecular Weight: 50 kDa
Observed Molecular Weight: 50 kDa
GenBank Accession Number: BC006520
Gene Symbol: EEF1G
Gene ID (NCBI): 1937
RRID: AB_2923672
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purification
UNIPROT ID: P26641
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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