Proteintech, 68194-1-Ig, NNT Monoclonal antibody

Size: 20ul / 150ul The NNT (68194-1-Ig) by Proteintech is a Monoclonal antibody targeting NNT in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to Human, Mouse, Rat, Rabbit, Pig samples 68194-1-Ig targets NNT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples. Tested Applications Positive WB detected in: pig heart tissue, LNCaP cells, HeLa cells, Jurkat cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat liver tissue, mouse liver tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human liver tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037). Specification Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig Cited Reactivity: mouse, rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4259 Product name: Recombinant human NNT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 30-236 aa of BC032370 Sequence: MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK Predict reactive species Full Name: nicotinamide nucleotide transhydrogenase Calculated Molecular Weight: 1085 aa, 114 kDa Observed Molecular Weight: 114 kDa GenBank Accession Number: BC032370 Gene Symbol: NNT Gene ID (NCBI): 23530 RRID: AB_2935283 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13423 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.