Proteintech, 68208-1-Ig, LETM1 Monoclonal antibody

Size: 20ul / 150ul The LETM1 (68208-1-Ig) by Proteintech is a Monoclonal antibody targeting LETM1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 68208-1-Ig targets LETM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, rat heart tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells Positive IHC detected in: rat adrenal gland tissue, mouse adrenal gland tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, U-251 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment. Specification Tested Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8851 Product name: Recombinant human LETM1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 391-739 aa of BC021208 Sequence: EDRLRGQLKQWLDLHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSEVAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS Predict reactive species Full Name: leucine zipper-EF-hand containing transmembrane protein 1 Calculated Molecular Weight: 739 aa, 83 kDa Observed Molecular Weight: 83 kDa GenBank Accession Number: BC021208 Gene Symbol: LETM1 Gene ID (NCBI): 3954 RRID: AB_2935296 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O95202 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.