Proteintech, 68224-1-Ig, CA12 Monoclonal antibody

Size: 20ul / 150ul The CA12 (68224-1-Ig) by Proteintech is a Monoclonal antibody targeting CA12 in WB, IF/ICC, ELISA applications with reactivity to Human samples 68224-1-Ig targets CA12 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: A549 cells, LNCaP cells, MCF-7 cells, HeLa cells Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution. Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7235 Product name: Recombinant human CA12 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 27-293 aa of BC000278 Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI Predict reactive species Full Name: carbonic anhydrase XII Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 38-45 kDa GenBank Accession Number: BC000278 Gene Symbol: CA12 Gene ID (NCBI): 771 RRID: AB_2935312 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O43570 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.