Proteintech, 68230-1-Ig, NKRF Monoclonal antibody

Size: 20ul / 150ul The NKRF (68230-1-Ig) by Proteintech is a Monoclonal antibody targeting NKRF in WB, ELISA applications with reactivity to Human, Rat, Mouse samples 68230-1-Ig targets NKRF in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples. Tested Applications Positive WB detected in: LNCaP cells, Jurkat cells, HEK-293 cells, MCF-7 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Specification Tested Reactivity: Human, Rat, Mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6374 Product name: Recombinant human NKRF protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 345-690 aa of BC068514 Sequence: NANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN Predict reactive species Full Name: NFKB repressing factor Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC068514 Gene Symbol: NKRF Gene ID (NCBI): 55922 RRID: AB_2935317 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O15226 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.