Proteintech, 68239-1-Ig, SNX15 Monoclonal antibody

Size: 20ul / 150ul The SNX15 (68239-1-Ig) by Proteintech is a Monoclonal antibody targeting SNX15 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 68239-1-Ig targets SNX15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8839 Product name: Recombinant human SNX15 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-342 aa of BC009897 Sequence: MSRQAKDDFLRHYTVSDPRTHPKGYTEYKVTAQFISKKDPEDVKEVVVWKRYSDFRKLHGDLAYTHRNLFRRLEEFPAFPRAQVFGRFEASVIEERRKGAEDLLRFTVHIPALNNSPQLKEFFRGGEVTRPLEVSRDLHILPPPLIPTPPPDDPRLSQLLPAERRGLEELEVPVDPPPSSPAQEALDLLFNCESTEEASGSPARGPLTEAELALFDPFSKEEGAAPSPTHVAELATMEVESARLDQEPWEPGGQEEEEDGEGGPTPAYLSQATELITQALRDEKAGAYAAALQGYRDGVHVLLQGVPSDPLPARQEGVKKKAAEYLKRAEEILRLHLSQLPP Predict reactive species Full Name: sorting nexin 15 Calculated Molecular Weight: 342 aa, 38 kDa Observed Molecular Weight: 40-50 kDa GenBank Accession Number: BC009897 Gene Symbol: SNX15 Gene ID (NCBI): 29907 RRID: AB_2935326 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NRS6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.