Proteintech, 68240-1-Ig, PSMA2 Monoclonal antibody

Size: 20ul / 150ul The PSMA2 (68240-1-Ig) by Proteintech is a Monoclonal antibody targeting PSMA2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig samples 68240-1-Ig targets PSMA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: U2OS cells, HepG2 cells, A431 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rat heart tissue, mouse heart tissue Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5837 Product name: Recombinant human PSMA2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-234 aa of BC047697 Sequence: MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDERSVHKVEPITKHIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQEYTQSGGVRPFGVSLLICGWNEGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNEDLELEDAIHTAILTLKESFEGQMTEDNIEVGICNEAGFRRLTPTEVKDYLAAIA Predict reactive species Full Name: proteasome (prosome, macropain) subunit, alpha type, 2 Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC047697 Gene Symbol: PSMA2 Gene ID (NCBI): 5683 RRID: AB_2935327 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P25787 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.