Proteintech, 68274-1-Ig, DHFR Monoclonal antibody

Size: 20ul / 150ul The DHFR (68274-1-Ig) by Proteintech is a Monoclonal antibody targeting DHFR in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 68274-1-Ig targets DHFR in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: LNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276). Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7318 Product name: Recombinant human DHFR protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-187 aa of BC000192 Sequence: MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND Predict reactive species Full Name: dihydrofolate reductase Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC000192 Gene Symbol: DHFR Gene ID (NCBI): 1719 RRID: AB_2935357 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P00374 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.