Proteintech, 68301-1-Ig, 5-methylcytosine Monoclonal antibody

Size: 20ul / 150ul The 5-methylcytosine (68301-1-Ig) by Proteintech is a Monoclonal antibody targeting 5-methylcytosine in IHC, Dot Blot, ELISA applications with reactivity to 5mc, chemical compound, m5c samples 68301-1-Ig targets 5-methylcytosine in WB, IHC, IF, RIP, Dot Blot, ELISA applications and shows reactivity with 5mc, chemical compound, m5c samples. Tested Applications Positive IHC detected in: mouse testis tissue, rat brain tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive ELISA detected in: compound Positive Dot Blot detected in: HeLa cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:2500-1:10000 Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100 DOT BLOT: DOT BLOT : 1:2500-1:10000 Background Information In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216). This antibody can recognize both 5mC in DNA and m5C in RNA.Protocol for Dot Blot:https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf Specification Tested Reactivity: 5mc, chemical compound, m5c Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Compound Predict reactive species Full Name: 5-methylcytosine RRID: AB_2935380 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.