Proteintech, 68305-1-Ig, SNX18 Monoclonal antibody

Size: 20ul / 150ul The SNX18 (68305-1-Ig) by Proteintech is a Monoclonal antibody targeting SNX18 in WB, IF/ICC, ELISA applications with reactivity to Human, Mouse samples 68305-1-Ig targets SNX18 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: HCT 116 cells, NIH/3T3 cells, A549 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695). Specification Tested Reactivity: Human, Mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17388 Product name: Recombinant human SNX18 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 55-284 aa of BC060791 Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT Predict reactive species Full Name: sorting nexin 18 Calculated Molecular Weight: 628 aa, 69 kDa Observed Molecular Weight: 67-70 kDa GenBank Accession Number: BC060791 Gene Symbol: SNX18 Gene ID (NCBI): 112574 RRID: AB_2935384 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q96RF0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.