Proteintech, 68307-1-Ig, MED23 Monoclonal antibody

Size: 20ul / 150ul The MED23 (68307-1-Ig) by Proteintech is a Monoclonal antibody targeting MED23 in WB, ELISA applications with reactivity to Human, Mouse, Rat samples 68307-1-Ig targets MED23 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: A549 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Specification Tested Reactivity: Human, Mouse, Rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28483 Product name: Recombinant human MED23 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-328 aa of BC060759 Sequence: METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQWIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQ Predict reactive species Full Name: mediator complex subunit 23 Calculated Molecular Weight: 156 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC060759 Gene Symbol: MED23 Gene ID (NCBI): 9439 RRID: AB_2935386 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9ULK4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.