Product Description
Size: 20ul / 150ul
The PBLD (68317-1-Ig) by Proteintech is a Monoclonal antibody targeting PBLD in WB, IF/ICC, ELISA applications with reactivity to Human, pig samples
68317-1-Ig targets PBLD in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.
Tested Applications
Positive WB detected in: pig liver tissue, pig kidney tissue
Positive IF/ICC detected in: HEK-293 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)
Specification
Tested Reactivity: Human, pig
Cited Reactivity: human, mouse
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag23827 Product name: Recombinant human PBLD protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-288 aa of BC009738 Sequence: MKLPIFIADAFTARAFRGNPAAVCLLENELDEDMHQKIAREMNLSETAFIRKLHPTDNFAQSSCFGLRWFTPASEVPLCGHATLASAAVLFHKIKNMNSTLTFVTLSGELRARRAEDGIVLDLPLYPAHPQDFHEVEDLIKTAIGNTLVQDICYSPDTQKLLVRLSDVYNRSFLENLKVNTENLLQVENTGKVKGLILTLKGEPGGQTQAFDFYSRYFAPWVGVAEDPVTGSAHAVLSSYWSQHLGKKEMHAFQCSHRGGELGISLRPDGRVDIRGGAAVVLEGTLTA Predict reactive species
Full Name: phenazine biosynthesis-like protein domain containing
Observed Molecular Weight: 30 kDa
GenBank Accession Number: BC009738
Gene Symbol: PBLD
Gene ID (NCBI): 64081
RRID: AB_3085053
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein G purification
UNIPROT ID: P30039
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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