Proteintech, 68395-1-Ig, Beta-2-Microglobulin Monoclonal antibody

Size: 20ul / 150ul The Beta-2-Microglobulin (68395-1-Ig) by Proteintech is a Monoclonal antibody targeting Beta-2-Microglobulin in IF/ICC, FC, ELISA applications with reactivity to human samples 68395-1-Ig targets Beta-2-Microglobulin in IF/ICC, FC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: NCCIT cells, A431 cells Positive FC detected in: Jurkat cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Flow Cytometry (FC): FC : 1.00 ug per 10^6 cells in a 100 µl suspension Background Information Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Eg31815 Product name: Recombinant Human Beta-2-Microglobulin protein (Myc Tag, His Tag) Source: mammalian cells -derived, pHZ-KIsec Tag: Myc & 6*His Domain: 21-119 aa of BC032589 Sequence: IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM Predict reactive species Full Name: beta-2-microglobulin Calculated Molecular Weight: 119 aa, 14 kDa Observed Molecular Weight: 12-14 kDa GenBank Accession Number: BC032589 Gene Symbol: B2M Gene ID (NCBI): 567 ENSEMBL Gene ID: ENSG00000166710 RRID: AB_3085112 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P61769 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.