Proteintech, 68396-1-Ig, ALDOB Monoclonal antibody

Size: 20ul / 150ul The ALDOB (68396-1-Ig) by Proteintech is a Monoclonal antibody targeting ALDOB in WB, IF/ICC, ELISA applications with reactivity to Human, mouse, rat, rabbit samples 68396-1-Ig targets ALDOB in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples. Tested Applications Positive WB detected in: rat kidney tissue, human urine sample, rabbit liver tissue, rat liver tissue, mouse liver tissue, mouse kidney tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA. Specification Tested Reactivity: Human, mouse, rat, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag12823 Product name: Recombinant human ALDOB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-316 aa of BC029399 Sequence: MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLPIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSGVARLQTRRQPRRLL Predict reactive species Full Name: aldolase B, fructose-bisphosphate Calculated Molecular Weight: 316 aa, 35 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC029399 Gene Symbol: ALDOB Gene ID (NCBI): 229 RRID: AB_3085113 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05062 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.