Proteintech, 68436-1-Ig, CNOT3 Monoclonal antibody

Size: 20ul / 150ul The CNOT3 (68436-1-Ig) by Proteintech is a Monoclonal antibody targeting CNOT3 in WB, ELISA applications with reactivity to Human, pig , mouse, rat , rabbit samples 68436-1-Ig targets CNOT3 in WB, ELISA applications and shows reactivity with Human, pig , mouse, rat , rabbit samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759]. Specification Tested Reactivity: Human, pig , mouse, rat , rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag33646 Product name: Recombinant human CNOT3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 449-753 aa of BC016474 Sequence: NASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ Predict reactive species Full Name: CCR4-NOT transcription complex, subunit 3 Calculated Molecular Weight: 120 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC016474 Gene Symbol: CNOT3 Gene ID (NCBI): 4849 RRID: AB_3085149 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O75175 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.