Proteintech, 68440-1-Ig, ATP6V1A Monoclonal antibody

Size: 20ul / 150ul The ATP6V1A (68440-1-Ig) by Proteintech is a Monoclonal antibody targeting ATP6V1A in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 68440-1-Ig targets ATP6V1A in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:40000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, mouse, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10820 Product name: Recombinant human ATP6V1A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 268-617 aa of BC013138 Sequence: NSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED Predict reactive species Full Name: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A Calculated Molecular Weight: 617 aa, 68 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC013138 Gene Symbol: ATP6V1A Gene ID (NCBI): 523 RRID: AB_3085153 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P38606 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.