Proteintech, 68510-1-Ig, CDCA8 Monoclonal antibody

Size: 20ul / 150ul The CDCA8 (68510-1-Ig) by Proteintech is a Monoclonal antibody targeting CDCA8 in WB, IF/ICC, ELISA applications with reactivity to human samples 68510-1-Ig targets CDCA8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MDA-MB-468 cells, A431 cells, HEK-293 cell, K-562 cells, Ramos cells, Daudi cells Positive IF/ICC detected in: A549 cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag3136 Product name: Recombinant human CDCA8 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-280 aa of BC016944 Sequence: MAPRKGSSRVANTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK Predict reactive species Full Name: cell division cycle associated 8 Calculated Molecular Weight: 280 aa, 31 kDa Observed Molecular Weight: 31-38 kDa GenBank Accession Number: BC016944 Gene Symbol: CDCA8 Gene ID (NCBI): 55143 RRID: AB_3085219 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q53HL2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.