Proteintech, 68517-1-Ig, AFG3L2 Monoclonal antibody

Size: 20ul / 150ul The AFG3L2 (68517-1-Ig) by Proteintech is a Monoclonal antibody targeting AFG3L2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 68517-1-Ig targets AFG3L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, Pig heart tissue, Rabbit heart tissue, Rat heart tissue, Mouse heart tissue Positive IF/ICC detected in: MCF-7 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5). Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7180 Product name: Recombinant human AFG3L2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 449-797 aa of BC065016 Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN Predict reactive species Full Name: AFG3 ATPase family gene 3-like 2 (yeast) Calculated Molecular Weight: 88 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: BC065016 Gene Symbol: AFG3L2 Gene ID (NCBI): 10939 RRID: AB_3085226 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y4W6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.