Proteintech, 68529-1-Ig, UQCRFS1 Monoclonal antibody

Size: 20ul / 150ul The UQCRFS1 (68529-1-Ig) by Proteintech is a Monoclonal antibody targeting UQCRFS1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 68529-1-Ig targets UQCRFS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13307 Product name: Recombinant human UQCRFS1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-274 aa of BC010035 Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG Predict reactive species Full Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC010035 Gene Symbol: UQCRFS1 Gene ID (NCBI): 7386 RRID: AB_3085237 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P47985 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.