Product Description
Size: 20ul / 150ul
The PUF60 (68533-1-Ig) by Proteintech is a Monoclonal antibody targeting PUF60 in WB, ELISA applications with reactivity to Human, mouse, rat, rabbit samples
68533-1-Ig targets PUF60 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
Tested Applications
Positive WB detected in: MCF-7 cells, mouse testis tissue
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Background Information
Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.
Specification
Tested Reactivity: Human, mouse, rat, rabbit
Cited Reactivity: mouse
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag33930 Product name: Recombinant human PUF60 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 243-542 aa of BC008875 Sequence: DPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA Predict reactive species
Full Name: poly-U binding splicing factor 60KDa
Calculated Molecular Weight: 60 kDa
Observed Molecular Weight: 60 kDa
GenBank Accession Number: BC008875
Gene Symbol: PUF60
Gene ID (NCBI): 22827
RRID: AB_3085241
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein G purification
UNIPROT ID: Q9UHX1
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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