Proteintech, 68535-1-Ig, UCHL5 Monoclonal antibody

Size: 20ul / 150ul The UCHL5 (68535-1-Ig) by Proteintech is a Monoclonal antibody targeting UCHL5 in WB, ELISA applications with reactivity to Human samples 68535-1-Ig targets UCHL5 in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HCT 116 cells, LNCaP cells, MCF-7 cells, HeLa cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935). Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: ubiquitin carboxyl-terminal hydrolase L5 Calculated Molecular Weight: 326 aa, 37 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: BC025369 Gene Symbol: UCHL5 Gene ID (NCBI): 51377 RRID: AB_3085243 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y5K5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.