Proteintech, 68536-1-Ig, EXO1/Exonuclease 1 Monoclonal antibody

Size: 20ul / 150ul The EXO1/Exonuclease 1 (68536-1-Ig) by Proteintech is a Monoclonal antibody targeting EXO1/Exonuclease 1 in WB, ELISA applications with reactivity to human samples 68536-1-Ig targets EXO1/Exonuclease 1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SH-SY5Y cells, HeLa cells, HEK-293T cells, MOLT-4 cells, TF-1 cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9253 Product name: Recombinant human EXO1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 496-846 aa of BC007491 Sequence: AVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREGACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLCLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ Predict reactive species Full Name: exonuclease 1 Calculated Molecular Weight: 94 kDa Observed Molecular Weight: 115-120 kDa GenBank Accession Number: BC007491 Gene Symbol: EXO1 Gene ID (NCBI): 9156 RRID: AB_3085244 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UQ84 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.