Proteintech, 68597-1-Ig, HOMER1 Monoclonal antibody

Size: 20ul / 150ul The HOMER1 (68597-1-Ig) by Proteintech is a Monoclonal antibody targeting HOMER1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit, chicken samples 68597-1-Ig targets HOMER1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples. Tested Applications Positive WB detected in: pig brain tissue, LNCaP cells, Calu-3 cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, MCF-10A cells, MOLT-4 cells, rat brain tisue, chicken brain tissue Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd. Specification Tested Reactivity: human, mouse, rat, pig, rabbit, chicken Cited Reactivity: mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag21302 Product name: Recombinant human HOMER1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 7-354 aa of BC015502 Sequence: FSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMTFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPTQNALPFSHSSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVTELECVSSQANAVHTHKTELNQTIQELEETLKLKEEEIERLKQEIDNARELQEQRDSLTQKLQEVEIRNKDLEGQLSDLEQRLEKSQNEQEAFRNNLKTLLEILDGKIFELTELRDNLAKLLECS Predict reactive species Full Name: homer homolog 1 (Drosophila) Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 40-46 kDa GenBank Accession Number: BC015502 Gene Symbol: HOMER1 Gene ID (NCBI): 9456 RRID: AB_3085295 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q86YM7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.