Proteintech, 68825-1-Ig, Mono/Di-Methyl-Histone H3 (Lys9) Monoclonal antibody

Size: 20ul / 150ul The Mono/Di-Methyl-Histone H3 (Lys9) (68825-1-Ig) by Proteintech is a Monoclonal antibody targeting Mono/Di-Methyl-Histone H3 (Lys9) in WB, ELISA applications with reactivity to Human, mouse, rat samples 68825-1-Ig targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, LNCaP cells, Jurkat cells, MOLT-4 cells, K-562 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2. Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 17 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 RRID: AB_3670436 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.