Proteintech, 68898-3-Ig, ICT1 Monoclonal antibody

Size: 20ul / 150ul The ICT1 (68898-3-Ig) by Proteintech is a Monoclonal antibody targeting ICT1 in WB, IF/ICC, ELISA applications with reactivity to human samples 68898-3-Ig targets ICT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HCT 116 cells, Calu-3 cells, SW480 cells, LNCaP cells, HeLa cells, HEK-293 cells, SK-BR-3 cells Positive IF/ICC detected in: Caco-2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag33109 Product name: Recombinant human ICT1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-206 aa of BC015335 Sequence: MAATRCLRWGLSRAGVWLLPPPARCPRRALHKQKDGTEFKSIYSLDKLYPESQGSDTAWRVPNGAKQADSDIPLDRLTISYCRSSGPGGQNVNKVNSKAEVRFHLATAEWIAEPVRQKIAITHKNKINRLGELILTSESSRYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERLRQKRIHSAVKTSRRVDMD Predict reactive species Full Name: immature colon carcinoma transcript 1 Calculated Molecular Weight: 24 kDa Observed Molecular Weight: 21-24 kDa GenBank Accession Number: BC015335 Gene Symbol: ICT1 Gene ID (NCBI): 3396 RRID: AB_3670448 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q14197 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.