Proteintech, 68999-1-Ig, Phospho-IkB Alpha (Ser32/36) Monoclonal antibody

Size: 20ul / 100ul The Phospho-IkB Alpha (Ser32/36) (68999-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-IkB Alpha (Ser32/36) in WB, ELISA applications with reactivity to human samples 68999-1-Ig targets Phospho-IkB Alpha (Ser32/36) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells, MCF-7 cells, Calyculin A treated MCF-7 cells, Calyculin A treated PC cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha). Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 36-39 kDa GenBank Accession Number: BC004983 Gene Symbol: IkB Alpha Gene ID (NCBI): 4792 RRID: AB_3670459 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P25963 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.